Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
Primary bone marrow-derived macrophages (BMDM)
strain
C57BL/6
cell type
BMDM cells (7th day of differentiation)
antibody
Anti-PU.1 (in-house rabbit polyclonal against the N-Terminal (aa. 1-100))
treatment
IL4 (10 ng/ml) for 4hrs

Sequenced DNA Library

library_name
GSM940929: PU.1_IL4_4h
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
38221917
Reads aligned (%)
99.4
Duplicates removed (%)
25.7
Number of peaks
104648 (qval < 1E-05)

mm9

Number of total reads
38221917
Reads aligned (%)
99.3
Duplicates removed (%)
25.8
Number of peaks
104693 (qval < 1E-05)

Base call quality data from DBCLS SRA