Sample information curated by ChIP-Atlas

Antigen

Antigen Class
DNase-seq
Antigen
DNase-Seq

Cell type

Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
U937
cell line
U937
cell type
acute myeloid leukemia (AML) cells
transduced with
constructs expressing mir-194-5p

Sequenced DNA Library

library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
DNA was prepared by isolating nuclei and incubating them with DNaseI before DNA isolation. DNaseI-seq libraries were prepared according to Illumina's standard protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
73451538
Reads aligned (%)
96.6
Duplicates removed (%)
4.9
Number of peaks
9492 (qval < 1E-05)

hg19

Number of total reads
73451538
Reads aligned (%)
96.0
Duplicates removed (%)
5.9
Number of peaks
8696 (qval < 1E-05)

Base call quality data from DBCLS SRA