Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Spermatocytes
MeSH Description
Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Attributes by original data submitter

Sample

source_name
spermatocytes
cell type
spermatocytes
age
15 days post-partum
strain
C57BL/6J
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
96911318
Reads aligned (%)
96.4
Duplicates removed (%)
23.0
Number of peaks
309 (qval < 1E-05)

mm9

Number of total reads
96911318
Reads aligned (%)
96.3
Duplicates removed (%)
22.9
Number of peaks
277 (qval < 1E-05)

Base call quality data from DBCLS SRA