Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
NF-E2_(SC-22827)
antibody antibodydescription
Rabbit Polyclonal, epitope: 1-230 mapping at the N-terminus of NF-E2 of human origin. Antibody Target: NF-E2
antibody targetdescription
Transcription factor NF-E2 45 kDa subunit is a component of the NF-E2 complex and essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2)recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. NFE2 has been shown to interact with CREB binding protein.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-22827
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000625
replicate
1

Sequenced DNA Library

library_name
GSM935652: Stanford_ChipSeq_GM12878_NF-E2_(SC-22827)_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
46073432
Reads aligned (%)
70.8
Duplicates removed (%)
6.9
Number of peaks
2032 (qval < 1E-05)

hg38

Number of total reads
46073432
Reads aligned (%)
72.5
Duplicates removed (%)
5.9
Number of peaks
1720 (qval < 1E-05)

Base call quality data from DBCLS SRA