Antigen

Antigen Class

TFs and others

Antigen

NFE2

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

NF-E2_(SC-22827)

antibody antibodydescription

Rabbit Polyclonal, epitope: 1-230 mapping at the N-terminus of NF-E2 of human origin. Antibody Target: NF-E2

antibody targetdescription

Transcription factor NF-E2 45 kDa subunit is a component of the NF-E2 complex and essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2)recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. NFE2 has been shown to interact with CREB binding protein.

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-22827

control

std

control description

Standard input signal for most experiments.

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000625

replicate

1

Sequenced DNA Library

library_name

GSM935652: Stanford_ChipSeq_GM12878_NF-E2_(SC-22827)_std

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

46073432

Reads aligned (%)

77.0

Duplicates removed (%)

5.9

Number of peaks

1720 (qval < 1E-05)

hg19

Number of total reads

46073432

Reads aligned (%)

76.1

Duplicates removed (%)

6.9

Number of peaks

2032 (qval < 1E-05)