Antigen

Antigen Class

TFs and others

Antigen

IRF3

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

IRF3

antibody antibodydescription

Rabbit polyclonal; epitope corresponding to amino acids 1-425 of IRF-3 of human origin. Antibody Target: IRF3

antibody targetdescription

Mediates interferon-stimulated response element (ISRE) promoter activation. DsRNA leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE.

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-9082

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

controlid

wgEncodeEH000706

replicate

1

Sequenced DNA Library

library_name

GSM935651: Stanford_ChipSeq_GM12878_IRF3_IgG-mus

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

55107978

Reads aligned (%)

89.0

Duplicates removed (%)

8.0

Number of peaks

2009 (qval < 1E-05)

hg19

Number of total reads

55107978

Reads aligned (%)

88.0

Duplicates removed (%)

9.5

Number of peaks

2231 (qval < 1E-05)