Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF3

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
IRF3
antibody antibodydescription
Rabbit polyclonal; epitope corresponding to amino acids 1-425 of IRF-3 of human origin. Antibody Target: IRF3
antibody targetdescription
Mediates interferon-stimulated response element (ISRE) promoter activation. DsRNA leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-9082
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
wgEncodeEH000706
replicate
1

Sequenced DNA Library

library_name
GSM935651: Stanford_ChipSeq_GM12878_IRF3_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
55107978
Reads aligned (%)
79.6
Duplicates removed (%)
9.5
Number of peaks
2231 (qval < 1E-05)

hg38

Number of total reads
55107978
Reads aligned (%)
81.9
Duplicates removed (%)
8.0
Number of peaks
2009 (qval < 1E-05)

Base call quality data from DBCLS SRA