Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF3

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
IRF3
antibody antibodydescription
Rabbit polyclonal; epitope corresponding to amino acids 1-425 of IRF-3 of human origin. Antibody Target: IRF3
antibody targetdescription
Mediates interferon-stimulated response element (ISRE) promoter activation. DsRNA leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-9082
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001791
replicate
1

Sequenced DNA Library

library_name
GSM935650: Stanford_ChipSeq_HepG2_IRF3_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
51207813
Reads aligned (%)
70.8
Duplicates removed (%)
12.9
Number of peaks
1304 (qval < 1E-05)

hg38

Number of total reads
51207813
Reads aligned (%)
72.8
Duplicates removed (%)
11.3
Number of peaks
1112 (qval < 1E-05)

Base call quality data from DBCLS SRA