Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAX

Cell type

Cell type Class
Blood
Cell type
NB-4
Primary Tissue
Blood
Site of Extraction
Bone Marrow
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
NB4
biomaterial_provider
Refer to protocol documents for differing sources, UW: DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) ACC-207 http://www.dsmz.de/ or Yale (Stanford): National Cell Culture Center http://www.nccc.com/
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
NB4
cell organism
human
cell description
acute promyelocytic leukemia cell line. (PMID: 1995093)
cell karyotype
cancer
cell lineage
mesoderm
cell sex
U
treatment
None
treatment description
No special treatment or protocol applies
antibody
Max
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target: Max
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-197
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000617
replicate
1

Sequenced DNA Library

library_name
GSM935644: Stanford_ChipSeq_NB4_Max_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
67906928
Reads aligned (%)
77.7
Duplicates removed (%)
7.8
Number of peaks
66060 (qval < 1E-05)

hg38

Number of total reads
67906928
Reads aligned (%)
79.1
Duplicates removed (%)
6.8
Number of peaks
66289 (qval < 1E-05)

Base call quality data from DBCLS SRA