Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
Brg1
antibody antibodydescription
Brg-1 (G-7) is a mouse monoclonal antibody raised against amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin. Antibody Target: BRG1
antibody targetdescription
Brg1 (SMARCA4) is an ATPase subunit of the SWI/SNF chromatin-remodeling complex.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-17796
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
wgEncodeEH000726
labversion
remapped from hg18, Illumina 2.0, low confidence sequences excluded. Data paired with IgG_Control.
replicate
1

Sequenced DNA Library

library_name
GSM935633: Stanford_ChipSeq_K562_Brg1_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
33314406
Reads aligned (%)
80.9
Duplicates removed (%)
8.4
Number of peaks
3216 (qval < 1E-05)

hg38

Number of total reads
33314406
Reads aligned (%)
81.9
Duplicates removed (%)
8.0
Number of peaks
3240 (qval < 1E-05)

Base call quality data from DBCLS SRA