Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPARGC1A

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
forskolin
treatment description
low-glucose DMEM with 0.5% BSA supplemented with 1uM forskolin and 1mM pyruvate for 6h. (Snyder)
antibody
PGC1A
antibody antibodydescription
rabbit polyclonal IgG. Antibody Target: PGC1A
antibody targetdescription
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-13067
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000755
labversion
Illumina 2.0, low confidence sequences excluded. Data paired with HepG2_Forskolin_Control.
replicate
1

Sequenced DNA Library

library_name
GSM935623: Stanford_ChipSeq_HepG2_forskolin_PGC1A_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
14817791
Reads aligned (%)
84.7
Duplicates removed (%)
6.8
Number of peaks
608 (qval < 1E-05)

hg38

Number of total reads
14817791
Reads aligned (%)
86.0
Duplicates removed (%)
6.1
Number of peaks
540 (qval < 1E-05)

Base call quality data from DBCLS SRA