Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

Pol2(phosphoS2)

antibody antibodydescription

Rabbit polyclonal against peptide conjugated to KLH derived from within residues 1600 - 1700 of S. cerevisiae RNA polymerase II CTD repeat YSPTSPS, phosphorylated at S2. Antibody Target: POL2S2

antibody targetdescription

RNA polymerase II, large subunit- specific for phosphorylated C-terminal domain.

antibody vendorname

Abcam

antibody vendorid

ab5095

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

controlid

wgEncodeEH000706

replicate

1

Sequenced DNA Library

library_name

GSM935608: Stanford_ChipSeq_GM12878_Pol2(phosphoS2)_IgG-mus

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

45550664

Reads aligned (%)

87.8

Duplicates removed (%)

15.4

Number of peaks

12185 (qval < 1E-05)

hg19

Number of total reads

45550664

Reads aligned (%)

86.6

Duplicates removed (%)

15.8

Number of peaks

12690 (qval < 1E-05)