Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRF1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
BRF1
antibody antibodydescription
Rabbit polyclonal - Anti-BRF antibody 128 was raised by immunising rabbits with synthetic peptide KISSKINYSVLRGLS (human BRF residues 533-547) coupled to keyhole limpet haemocyanin. Antibody Target: BRF1
antibody targetdescription
'B-related factor 1', subunit of RNA polymerase III transcription initiation factor IIIB
antibody vendorname
Donated by Dr. RJ White, The Beatson Institute for Cancer Research
antibody vendorid
128
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000615
labversion
Illumina 2.0, low confidence sequences excluded, fragmented with both a probe sonicator and a Misonix sonicator, and precipitated with protein A sepharose beads
replicate
1

Sequenced DNA Library

library_name
GSM935595: Harvard_ChipSeq_K562_BRF1_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
18469470
Reads aligned (%)
83.0
Duplicates removed (%)
7.1
Number of peaks
725 (qval < 1E-05)

hg38

Number of total reads
18469470
Reads aligned (%)
84.3
Duplicates removed (%)
6.3
Number of peaks
509 (qval < 1E-05)

Base call quality data from DBCLS SRA