SRX150667
GSM935588: Harvard ChipSeq MCF10A-Er-Src EtOH 0.01pct Pol2 std

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A-Er-Src
biomaterial_provider
Struhl laboratory
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MCF10A-Er-Src
cell organism
human
cell description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
cell karyotype
normal
cell lineage
ectoderm
cell sex
F
treatment
EtOH_0.01pct
treatment description
36 h with 0.01% Ethanol (Snyder)
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001782
replicate
1

Sequenced DNA Library

library_name
GSM935588: Harvard_ChipSeq_MCF10A-Er-Src_EtOH_0.01pct_Pol2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
69172664
Reads aligned (%)
87.7
Duplicates removed (%)
35.0
Number of peaks
28439 (qval < 1E-05)

hg19

Number of total reads
69172664
Reads aligned (%)
86.5
Duplicates removed (%)
35.3
Number of peaks
28785 (qval < 1E-05)

Base call quality data from DBCLS SRA