Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GTF2F1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H1-hESC
cell organism
human
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
GTF2F1_(AB28179)
antibody antibodydescription
Rabbit polyclonal to TFIIF - Recombinant full length protein - RAP 74 subunit of TF11F. Antibody Target: GTF2F1
antibody targetdescription
RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
antibody vendorname
Abcam
antibody vendorid
ab28179
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001834
replicate
1

Sequenced DNA Library

library_name
GSM935581: Stanford_ChipSeq_H1-hESC_GTF2F1_(AB28179)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54896984
Reads aligned (%)
89.5
Duplicates removed (%)
6.3
Number of peaks
2733 (qval < 1E-05)

hg38

Number of total reads
54896984
Reads aligned (%)
91.3
Duplicates removed (%)
5.1
Number of peaks
4417 (qval < 1E-05)

Base call quality data from DBCLS SRA