Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RCOR1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
COREST_(sc-30189)
antibody antibodydescription
Rabbit polyclonal, epitope corresponding to amino acids 246-310 mapping within an internal region of CoREST of human origin recommended for detection of CoREST of mouse, rat and human origin. Antibody Target: COREST (sc30189)
antibody targetdescription
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-30189
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000744
replicate
1

Sequenced DNA Library

library_name
GSM935578: Stanford_ChipSeq_HeLa-S3_COREST_(sc-30189)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
57449978
Reads aligned (%)
89.8
Duplicates removed (%)
7.0
Number of peaks
27956 (qval < 1E-05)

hg38

Number of total reads
57449978
Reads aligned (%)
91.6
Duplicates removed (%)
5.9
Number of peaks
28344 (qval < 1E-05)

Base call quality data from DBCLS SRA