Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RFX5

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
RFX5_(200-401-194)
antibody antibodydescription
Rabbit polyclonal against RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin. Antibody Target: RFX5
antibody targetdescription
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. RFX binds the X1 box of MHC-II promoters
antibody vendorname
Rockland Immunochemicals
antibody vendorid
200-401-194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935565: Stanford_ChipSeq_K562_RFX5_(200-401-194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
58491650
Reads aligned (%)
79.4
Duplicates removed (%)
15.0
Number of peaks
2853 (qval < 1E-05)

hg38

Number of total reads
58491650
Reads aligned (%)
81.0
Duplicates removed (%)
13.9
Number of peaks
2855 (qval < 1E-05)

Base call quality data from DBCLS SRA