Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF217

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MCF-7
cell organism
human
cell description
mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
ZNF217
antibody antibodydescription
A rabbit polyclonal antibody raised against a recombinant ZNF217 protein corresponding to the 1562-2207 nucleotides from a human ZNF217 cDNA. Antibody Target: ZNF217
antibody targetdescription
ZNF217 is an important oncogene based on the high frequency of amplification and overexpression in many cancer types, but its molecular mode of gene regulation is poorly understood. ZNF217 has been purified in complexes that contain repressor proteins such as CtBP2, suggesting that it acts as a transcriptional repressor. ZNF217 contains seven C2H2 zinc finger domains and has been shown to bind to gene promoters
antibody vendorname
From the lab of Dr. Paul Yaswen
antibody vendorid
ZNF217
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000762
replicate
1

Sequenced DNA Library

library_name
GSM935563: USC_ChipSeq_MCF-7_ZNF217_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
58141429
Reads aligned (%)
86.8
Duplicates removed (%)
7.9
Number of peaks
11488 (qval < 1E-05)

hg38

Number of total reads
58141429
Reads aligned (%)
88.5
Duplicates removed (%)
6.8
Number of peaks
11368 (qval < 1E-05)

Base call quality data from DBCLS SRA