Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RFX5

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
RFX5_(200-401-194)
antibody antibodydescription
Rabbit polyclonal against RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin. Antibody Target: RFX5
antibody targetdescription
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. RFX binds the X1 box of MHC-II promoters
antibody vendorname
Rockland Immunochemicals
antibody vendorid
200-401-194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000744
replicate
1

Sequenced DNA Library

library_name
GSM935560: Stanford_ChipSeq_HeLa-S3_RFX5_(200-401-194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
67477197
Reads aligned (%)
66.8
Duplicates removed (%)
9.5
Number of peaks
38034 (qval < 1E-05)

hg38

Number of total reads
67477197
Reads aligned (%)
68.0
Duplicates removed (%)
8.6
Number of peaks
38257 (qval < 1E-05)

Base call quality data from DBCLS SRA