Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
p300_(SC-584)
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the N-terminus of p300 of human origin. Antibody Target: P300
antibody targetdescription
Encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-584
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
wgEncodeEH000706
replicate
1

Sequenced DNA Library

library_name
GSM935559: Stanford_ChipSeq_GM12878_p300_(SC-584)_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
55100858
Reads aligned (%)
68.6
Duplicates removed (%)
9.0
Number of peaks
12760 (qval < 1E-05)

hg38

Number of total reads
55100858
Reads aligned (%)
70.2
Duplicates removed (%)
7.8
Number of peaks
12705 (qval < 1E-05)

Base call quality data from DBCLS SRA