Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GTF2F1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
GTF2F1_(AB28179)
antibody antibodydescription
Rabbit polyclonal to TFIIF - Recombinant full length protein - RAP 74 subunit of TF11F. Antibody Target: GTF2F1
antibody targetdescription
RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
antibody vendorname
Abcam
antibody vendorid
ab28179
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000744
replicate
1

Sequenced DNA Library

library_name
GSM935554: Stanford_ChipSeq_HeLa-S3_GTF2F1_(AB28179)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
60397010
Reads aligned (%)
68.3
Duplicates removed (%)
3.9
Number of peaks
20475 (qval < 1E-05)

hg19

Number of total reads
60397010
Reads aligned (%)
67.8
Duplicates removed (%)
5.0
Number of peaks
20170 (qval < 1E-05)

Base call quality data from DBCLS SRA