Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A-Er-Src
biomaterial_provider
Struhl laboratory
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MCF10A-Er-Src
cell organism
human
cell description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
cell karyotype
normal
cell lineage
ectoderm
cell sex
F
treatment
EtOH_0.01pct_12hr
treatment description
12 h with 0.01% Ethanol (Snyder)
antibody
STAT3
antibody antibodydescription
Rabbit polyclonal; epitope mapping at the C-terminus of Stat3 of mouse origin. Antibody Target: STAT3
antibody targetdescription
Member of STAT family. After phosphorylation in response to cytokines and growth factors (including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2), forms homo- or heterodimers that translocate to nucleus and activate transcription. Activates expression of a variety of genes with roles in processes such as cell growth and apoptosis.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-482
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001869
replicate
1

Sequenced DNA Library

library_name
GSM935551: Harvard_ChipSeq_MCF10A-Er-Src_EtOH_0.01pct_12hr_STAT3_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
42446970
Reads aligned (%)
61.9
Duplicates removed (%)
25.1
Number of peaks
47688 (qval < 1E-05)

hg38

Number of total reads
42446970
Reads aligned (%)
63.1
Duplicates removed (%)
24.2
Number of peaks
47711 (qval < 1E-05)

Base call quality data from DBCLS SRA