Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CCNT2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
CCNT2
antibody antibodydescription
Goal polyclonal. Raised against an internal region of human CycT2a/b. Recommeded for detection of CycT2a/b of rat, mouse or human origin. Vendor recommeds it use for the folloiwng applications: WB, IF and ELISA. Antibody Target: CCNT2
antibody targetdescription
CycT2 is one of three cyclins that can form a heterodimer with cyclin-dependent kinase 9 (CDK9). The heterodimer is known as positive elongation factor b (P-TEFb) and is responsible for the phosphophorylation of Ser2 of the heptad repeat in the C-terminal domain of RNA polymerase II as well as the negative elongation factors DSIF (hSpt4/hSpt5) and NELF. Phosphorylation of RNA polymerase II and the negative elongation factors by P-TEFb promotes elongation. CycT2 contains a leucine rich domain capable of binding to the C-terminal domain of RNA polymerase II.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-12419
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000615
replicate
1

Sequenced DNA Library

library_name
GSM935547: Harvard_ChipSeq_K562_CCNT2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
37363313
Reads aligned (%)
57.8
Duplicates removed (%)
23.4
Number of peaks
19673 (qval < 1E-05)

hg38

Number of total reads
37363313
Reads aligned (%)
59.0
Duplicates removed (%)
22.4
Number of peaks
19691 (qval < 1E-05)

Base call quality data from DBCLS SRA