Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
IFNa30
treatment description
30 m of Interferon alpha (Snyder)
antibody
IRF1
antibody antibodydescription
IRF-1 (C-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of IRF-1 of human origin. Antibody Target: IRF1
antibody targetdescription
Interferon regulatory factor 1 is a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription and also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. IRF1 has been shown to play roles in regulating apoptosis and tumor suppression.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-497
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000655
replicate
1

Sequenced DNA Library

library_name
GSM935546: Stanford_ChipSeq_K562_IFNa30_IRF1_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
35140474
Reads aligned (%)
72.4
Duplicates removed (%)
5.5
Number of peaks
1656 (qval < 1E-05)

hg38

Number of total reads
35140474
Reads aligned (%)
74.0
Duplicates removed (%)
4.3
Number of peaks
2081 (qval < 1E-05)

Base call quality data from DBCLS SRA