Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
p300_(SC-584)
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the N-terminus of p300 of human origin. Antibody Target: P300
antibody targetdescription
Encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-584
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001791
replicate
1

Sequenced DNA Library

library_name
GSM935545: Stanford_ChipSeq_HepG2_p300_(SC-584)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
52756547
Reads aligned (%)
86.0
Duplicates removed (%)
5.7
Number of peaks
8914 (qval < 1E-05)

hg38

Number of total reads
52756547
Reads aligned (%)
87.9
Duplicates removed (%)
4.5
Number of peaks
9074 (qval < 1E-05)

Base call quality data from DBCLS SRA