Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HMGN3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
HMGN3
antibody antibodydescription
Rabbit polyclonal. Raised against an amino acids 60 to 99 of human HMGN3. Vendor recommeds its use for detection of HMGN3 in human samples using immunohistochemistry. Antibody Target: HMGN3
antibody targetdescription
HMGN3 is a high-mobility group protein. These proteins are small, 8 to 11 kDa, ubiquitous proteins believed to play a role in chromatin structure. HMGNs bind to nucleosomes between the histone core and DNA and reduce chromatin compaction. As a result HMGNs, HMGN3 included, are believed to increase access to DNA for DNA replication, repair and transcription. HMGN3 has specifically been implicated in the action of Thyroid horomone receptors.
antibody vendorname
Novus Biologicals
antibody vendorid
NB100-61076
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000615
replicate
1

Sequenced DNA Library

library_name
GSM935544: Harvard_ChipSeq_K562_HMGN3_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
34396876
Reads aligned (%)
69.8
Duplicates removed (%)
11.1
Number of peaks
16076 (qval < 1E-05)

hg38

Number of total reads
34396876
Reads aligned (%)
70.7
Duplicates removed (%)
10.5
Number of peaks
16099 (qval < 1E-05)

Base call quality data from DBCLS SRA