Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Blood
Cell type
GM18951
Tissue
blood
Lineage
mesoderm
Description
lymphoblastoid, International HapMap Project, Japanese in Tokyo, Japan, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM18951
biomaterial_provider
Coriell
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM18951
cell organism
human
cell description
lymphoblastoid, International HapMap Project, Japanese in Tokyo, Japan, treatment: Epstein-Barr Virus transformed
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
TNFa
treatment description
human recombinant TNF-alpha from eBioscience [product# 14-8329-62] (Snyder)
antibody
NFKB
antibody antibodydescription
Rabbit polyclonal. Antibody Target: NFKB
antibody targetdescription
Epitope mapping at the C-terminus of NF-kappa-B p65 of human origin, recommended for detection of NFkB p65 of m, r and h origin by WB, IP, IF and ELISA
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-372
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000777
labversion
remapped from hg18, Illumina 2.0, low confidence sequences excluded. Data paired with GM18951_IgG_Control.
replicate
1

Sequenced DNA Library

library_name
GSM935531: Stanford_ChipSeq_GM18951_TNFa_NFKB_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
55924894
Reads aligned (%)
64.6
Duplicates removed (%)
3.7
Number of peaks
23192 (qval < 1E-05)

hg38

Number of total reads
55924894
Reads aligned (%)
66.1
Duplicates removed (%)
3.6
Number of peaks
22759 (qval < 1E-05)

Base call quality data from DBCLS SRA