Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Blood
Cell type
GM10847
Tissue
blood
Lineage
mesoderm
Description
lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM10847
biomaterial_provider
Coriell
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM10847
cell organism
human
cell description
lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus transformed
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
TNFa
treatment description
human recombinant TNF-alpha from eBioscience [product# 14-8329-62] (Snyder)
antibody
NFKB
antibody antibodydescription
Rabbit polyclonal. Antibody Target: NFKB
antibody targetdescription
Epitope mapping at the C-terminus of NF-kappa-B p65 of human origin, recommended for detection of NFkB p65 of m, r and h origin by WB, IP, IF and ELISA
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-372
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000770
labversion
remapped from hg18, Illumina 2.0, low confidence sequences excluded. Data paired with GM10847_IgG_Control
replicate
1

Sequenced DNA Library

library_name
GSM935527: Stanford_ChipSeq_GM10847_TNFa_NFKB_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68028072
Reads aligned (%)
66.3
Duplicates removed (%)
3.8
Number of peaks
18424 (qval < 1E-05)

hg38

Number of total reads
68028072
Reads aligned (%)
67.9
Duplicates removed (%)
7.5
Number of peaks
18251 (qval < 1E-05)

Base call quality data from DBCLS SRA