Antigen

Antigen Class

TFs and others

Antigen

RELA

Cell type

Cell type Class

Blood

Cell type

GM12891

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Sample

source_name

GM12891

biomaterial_provider

Coriell

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12891

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

cell karyotype

normal

cell lineage

mesoderm

cell sex

M

treatment

TNFa

treatment description

human recombinant TNF-alpha from eBioscience [product# 14-8329-62] (Snyder)

antibody

NFKB

antibody antibodydescription

Rabbit polyclonal. Antibody Target: NFKB

antibody targetdescription

Epitope mapping at the C-terminus of NF-kappa-B p65 of human origin, recommended for detection of NFkB p65 of m, r and h origin by WB, IP, IF and ELISA

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-372

control

IgG-rab

control description

Input signal from Normal Rabbit IgG ChIP-seq.

control

IgG-rab

control description

Input signal from Normal Rabbit IgG ChIP-seq.

controlid

wgEncodeEH000772

labversion

remapped from hg18, Data paired with GM12891_IgG_Control.

replicate

1

Sequenced DNA Library

library_name

GSM935526: Stanford_ChipSeq_GM12891_TNFa_NFKB_IgG-rab

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

81568731

Reads aligned (%)

61.4

Duplicates removed (%)

11.7

Number of peaks

44950 (qval < 1E-05)

hg19

Number of total reads

81568731

Reads aligned (%)

58.6

Duplicates removed (%)

9.9

Number of peaks

45981 (qval < 1E-05)