Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAFF

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
MafF_(M8194)
antibody antibodydescription
Rabbit polyclonal. Raised against synthetic peptide corresponding to amino acids 147-163 of human MAFF. The immunizing peptide does not cross-react with other members of the family. Antibody Target: MafF
antibody targetdescription
The protein encoded by this gene is a (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. Can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. May also be involved in the cellular stress response.
antibody vendorname
Sigma-Aldrich
antibody vendorid
M8194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935520: Stanford_ChipSeq_K562_MafF_(M8194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
56000395
Reads aligned (%)
88.5
Duplicates removed (%)
7.5
Number of peaks
30328 (qval < 1E-05)

hg38

Number of total reads
56000395
Reads aligned (%)
90.5
Duplicates removed (%)
6.1
Number of peaks
29641 (qval < 1E-05)

Base call quality data from DBCLS SRA