Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Yale
lab description
Snyder - Yale University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
Brg1
antibody antibodydescription
Brg-1 (G-7) is a mouse monoclonal antibody raised against amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin. Antibody Target: BRG1
antibody targetdescription
Brg1 (SMARCA4) is an ATPase subunit of the SWI/SNF chromatin-remodeling complex.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-17796
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
wgEncodeEH000633
labversion
remapped from hg18, Illumina 2.0, low confidence sequences excluded
replicate
1

Sequenced DNA Library

library_name
GSM935511: Yale_ChipSeq_HeLa-S3_Brg1_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
25454467
Reads aligned (%)
85.5
Duplicates removed (%)
5.7
Number of peaks
679 (qval < 1E-05)

hg38

Number of total reads
25454467
Reads aligned (%)
86.7
Duplicates removed (%)
5.1
Number of peaks
702 (qval < 1E-05)

Base call quality data from DBCLS SRA