Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF274

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
ZNF274_(M01)
antibody antibodydescription
Mouse monoclonal antibody raised against a partial recombinant ZNF274 (corresponding to amino acids 420-531 of the human ZNF274 protein). Antibody Target: ZNF274
antibody targetdescription
ZNF274 is a zinc finger protein containing five C2H2-type zinc finger domains, two Kruppel-associated box A (KRABA) domains, and a leucine-rich SCAN domain. The encoded protein has been suggested to be a transcriptional repressor and interacts with the KAP1 corepressor complex.
antibody vendorname
Abnova ZNF74
antibody vendorid
H00010782-M01
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000672
replicate
1

Sequenced DNA Library

library_name
GSM935503: USC_ChipSeq_K562_ZNF274_(M01)_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
66472291
Reads aligned (%)
59.8
Duplicates removed (%)
33.9
Number of peaks
2825 (qval < 1E-05)

hg38

Number of total reads
66472291
Reads aligned (%)
61.2
Duplicates removed (%)
32.9
Number of peaks
2834 (qval < 1E-05)

Base call quality data from DBCLS SRA