Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GTF2F1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
GTF2F1_(AB28179)
antibody antibodydescription
Rabbit polyclonal to TFIIF - Recombinant full length protein - RAP 74 subunit of TF11F. Antibody Target: GTF2F1
antibody targetdescription
RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
antibody vendorname
Abcam
antibody vendorid
ab28179
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935501: Stanford_ChipSeq_K562_GTF2F1_(AB28179)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
51436519
Reads aligned (%)
84.6
Duplicates removed (%)
6.1
Number of peaks
2398 (qval < 1E-05)

hg38

Number of total reads
51436519
Reads aligned (%)
86.5
Duplicates removed (%)
4.8
Number of peaks
3597 (qval < 1E-05)

Base call quality data from DBCLS SRA