Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
p300_(SC-584)
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the N-terminus of p300 of human origin. Antibody Target: P300
antibody targetdescription
Encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-584
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000744
replicate
1

Sequenced DNA Library

library_name
GSM935500: Stanford_ChipSeq_HeLa-S3_p300_(SC-584)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
61147610
Reads aligned (%)
62.0
Duplicates removed (%)
8.8
Number of peaks
31444 (qval < 1E-05)

hg38

Number of total reads
61147610
Reads aligned (%)
63.6
Duplicates removed (%)
7.4
Number of peaks
31574 (qval < 1E-05)

Base call quality data from DBCLS SRA