Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
p300_(SC-48343)
antibody antibodydescription
mouse monoclonal raised against amino acids 774-1045 of p300 of human origin. Antibody Target: P300
antibody targetdescription
Encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-48343
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935494: Stanford_ChipSeq_K562_p300_(SC-48343)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
59109525
Reads aligned (%)
69.4
Duplicates removed (%)
11.3
Number of peaks
2216 (qval < 1E-05)

hg38

Number of total reads
59109525
Reads aligned (%)
70.9
Duplicates removed (%)
10.3
Number of peaks
3662 (qval < 1E-05)

Base call quality data from DBCLS SRA