Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRF2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
BRF2
antibody antibodydescription
Antibody 4295 against Brf2 was raised by immunizing rabbits with keyhole limpet hemocyanin coupled to synthetic peptides VSRSQQRGLRRVRDLC and SDSEIEQYLRTPQEVR, corresponding to human Brf2 residues 66-80 and 385-400, respectively. Antibody Target: BRF2
antibody targetdescription
Brf2 is a component of an alternate form of the RNA Polymerase III transcription factor TFIIIB.
antibody vendorname
Donated by Dr. RJ White, The Beatson Institute for Cancer Research
antibody vendorid
4295
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000615
replicate
1

Sequenced DNA Library

library_name
GSM935490: Harvard_ChipSeq_K562_BRF2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
51487836
Reads aligned (%)
58.4
Duplicates removed (%)
7.7
Number of peaks
861 (qval < 1E-05)

hg38

Number of total reads
51487836
Reads aligned (%)
59.5
Duplicates removed (%)
6.7
Number of peaks
545 (qval < 1E-05)

Base call quality data from DBCLS SRA