Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
E2F1
antibody antibodydescription
E2F1(KH20 and KH95) is a mouse monoclonal antibody. The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386. Recommended for detection of E2F1 of H and Xn origin by WB and IP. Antibody Target: E2F1
antibody targetdescription
E2F1, the original member of the E2F family of transcription factors, was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. E2F1 is cell cycle regulated with very low levels in early G1, then increasing levels as cells move from G1 to S, and highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
antibody vendorname
Millipore
antibody vendorid
05-379
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000612
labversion
HeLa cells stably expressed a tagged HA-E2F1 were fragmented using Bioruptor, precipitated with StaphA and an antibody to E2F1,
replicate
1
softwareversion
PeakSeq1.0 (fdr 0.001)

Sequenced DNA Library

library_name
GSM935484: USC_ChipSeq_HeLa-S3_E2F1_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
50027676
Reads aligned (%)
69.4
Duplicates removed (%)
3.6
Number of peaks
10240 (qval < 1E-05)

hg38

Number of total reads
50027676
Reads aligned (%)
70.7
Duplicates removed (%)
2.5
Number of peaks
10147 (qval < 1E-05)

Base call quality data from DBCLS SRA