Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF274

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
ZNF274
antibody antibodydescription
Mouse polyclonal antibody raised against a partial recombinant ZNF274 (GST-fusion of a.a.420-531 of human ZNF274 protein). Works in IP, WB and ELISA. Antibody Target: ZNF274
antibody targetdescription
ZNF274 is a zinc finger protein containing five C2H2-type zinc finger domains, two Kruppel-associated box A (KRABA) domains, and a leucine-rich SCAN domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus.
antibody vendorname
ABNOVA
antibody vendorid
H00010782-A01
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000672
labversion
Fragmented using Bioruptor, precipitated with StaphA
replicate
1
softwareversion
PeakSeq1.0 (fdr 0.001)

Sequenced DNA Library

library_name
GSM935479: USC_ChipSeq_K562_ZNF274_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
70354669
Reads aligned (%)
33.1
Duplicates removed (%)
3.5
Number of peaks
1046 (qval < 1E-05)

hg38

Number of total reads
70354669
Reads aligned (%)
34.1
Duplicates removed (%)
1.9
Number of peaks
834 (qval < 1E-05)

Base call quality data from DBCLS SRA