Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Yale
lab description
Snyder - Yale University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
IFNa30
treatment description
30 m of Interferon alpha (Snyder)
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000655
labversion
Illumina 2.0, low confidence sequences excluded
replicate
1

Sequenced DNA Library

library_name
GSM935475: Yale_ChipSeq_K562_IFNa30_Pol2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
24160594
Reads aligned (%)
83.1
Duplicates removed (%)
4.4
Number of peaks
17444 (qval < 1E-05)

hg19

Number of total reads
24160594
Reads aligned (%)
82.4
Duplicates removed (%)
4.8
Number of peaks
17456 (qval < 1E-05)

Base call quality data from DBCLS SRA