Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BHLHE40

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
A549
cell organism
human
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
BHLHE40
antibody antibodydescription
Dec-1(S-8) is a mouse monoclonal IgG2b antibody raised against recombinant DEC1 of human origin. Recommended for detection of DEC1 of m and h origin by WB, IP and ELISA. Antibody Target: BHLHE40
antibody targetdescription
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differe
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101023
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH002819
replicate
1

Sequenced DNA Library

library_name
GSM935440: Stanford_ChipSeq_A549_BHLHE40_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
55109165
Reads aligned (%)
90.1
Duplicates removed (%)
6.9
Number of peaks
2967 (qval < 1E-05)

hg38

Number of total reads
55109165
Reads aligned (%)
92.0
Duplicates removed (%)
5.7
Number of peaks
3338 (qval < 1E-05)

Base call quality data from DBCLS SRA