Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOS

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A-Er-Src
biomaterial_provider
Struhl laboratory
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MCF10A-Er-Src
cell organism
human
cell description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
cell karyotype
normal
cell lineage
ectoderm
cell sex
F
treatment
EtOH_0.01pct
treatment description
36 h with 0.01% Ethanol (Snyder)
antibody
c-Fos
antibody antibodydescription
Rabbit polyclonal to amino acids 210-335 mapping at the C-terminus of c-Fos of human origin. Antibody Target: c-Fos
antibody targetdescription
Heterodimer of Fos and Jun constitute transcription factor AP1. Proto-oncogene c-Jun is a leucine-zipper.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-7202
control
Harvard_Control
control description
input library was prepared at Harvard.
control
Harvard_Control
control description
input library was prepared at Harvard.
controlid
wgEncodeEH002871
replicate
1

Sequenced DNA Library

library_name
GSM935438: Harvard_ChipSeq_MCF10A-Er-Src_EtOH_0.01pct_c-Fos_Harvard_Control
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
50667127
Reads aligned (%)
71.0
Duplicates removed (%)
23.9
Number of peaks
80828 (qval < 1E-05)

hg38

Number of total reads
50667127
Reads aligned (%)
72.3
Duplicates removed (%)
23.1
Number of peaks
81051 (qval < 1E-05)

Base call quality data from DBCLS SRA