Antigen

Antigen Class

TFs and others

Antigen

SUPT20H

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Harvard

lab description

Struhl - Harvard University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

SPT20

antibody antibodydescription

Rabbit polyclonal anti-SPT20 antibody (3006), Immunogen: C-terminal region of mSPT20 (330-531 aa) is cloned in pET28b and over expressed in E. Coli. Protein was purified on Ni-NTA exchange column. Sequence: mSPT20 (330-531 amino acids). Antibody Target: SPT20

antibody targetdescription

Also known as FAM48A or P38IP. It is a component of SAGA complex. Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail.

antibody vendorname

Laszlo Tora

antibody vendorid

missing

control

std

control description

Standard input signal for most experiments.

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000625

replicate

1

Sequenced DNA Library

library_name

GSM935417: Harvard_ChipSeq_GM12878_SPT20_std

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

63040797

Reads aligned (%)

85.9

Duplicates removed (%)

18.5

Number of peaks

1249 (qval < 1E-05)

hg19

Number of total reads

63040797

Reads aligned (%)

85.0

Duplicates removed (%)

19.7

Number of peaks

1435 (qval < 1E-05)