Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUPT20H

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
SPT20
antibody antibodydescription
Rabbit polyclonal anti-SPT20 antibody (3006), Immunogen: C-terminal region of mSPT20 (330-531 aa) is cloned in pET28b and over expressed in E. Coli. Protein was purified on Ni-NTA exchange column. Sequence: mSPT20 (330-531 amino acids). Antibody Target: SPT20
antibody targetdescription
Also known as FAM48A or P38IP. It is a component of SAGA complex. Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail.
antibody vendorname
Laszlo Tora
antibody vendorid
missing
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000625
replicate
1

Sequenced DNA Library

library_name
GSM935417: Harvard_ChipSeq_GM12878_SPT20_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
63040797
Reads aligned (%)
68.3
Duplicates removed (%)
19.7
Number of peaks
1435 (qval < 1E-05)

hg38

Number of total reads
63040797
Reads aligned (%)
70.1
Duplicates removed (%)
18.5
Number of peaks
1249 (qval < 1E-05)

Base call quality data from DBCLS SRA