Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KAT2A

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
GCN5
antibody antibodydescription
Rabbit polyclonal anti-GCN5 antibody (2676), Immunogen : synthetic peptide coupled to Ovalbumin (sequence-MAEPSQAPTPAPAAQPRPLC). Antibody Target: GCN5
antibody targetdescription
KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. Acetylation of histones gives a specific tag for epigenetic transcription activation. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the SAGA and ATAC complexes, complexes with histone acetyltransferase activities on histones H3 and H4
antibody vendorname
Laszlo Tora
antibody vendorid
Missing
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000625
replicate
1

Sequenced DNA Library

library_name
GSM935415: Harvard_ChipSeq_GM12878_GCN5_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
44912180
Reads aligned (%)
79.0
Duplicates removed (%)
7.7
Number of peaks
1806 (qval < 1E-05)

hg38

Number of total reads
44912180
Reads aligned (%)
80.6
Duplicates removed (%)
6.6
Number of peaks
1284 (qval < 1E-05)

Base call quality data from DBCLS SRA