Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F4

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A-Er-Src
biomaterial_provider
Struhl laboratory
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MCF10A-Er-Src
cell organism
human
cell description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
cell karyotype
normal
cell lineage
ectoderm
cell sex
F
treatment
4OHTAM_1uM_36hr
treatment description
(To replace TAM_1uM_36hr) 36 h with 1 uM 4-hydroxytamoxifen (Myers)
antibody
E2F4
antibody antibodydescription
rabbit polyclonal. Antibody Target: E2F4
antibody targetdescription
mapping at the C-terminus of E2F4 of human origin
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-866
control
Harvard_Control
control description
input library was prepared at Harvard.
control
Harvard_Control
control description
input library was prepared at Harvard.
controlid
wgEncodeEH002871
replicate
1

Sequenced DNA Library

library_name
GSM935400: Harvard_ChipSeq_MCF10A-Er-Src_4OHTAM_1uM_36hr_E2F4_Harvard_Control
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
61955386
Reads aligned (%)
60.4
Duplicates removed (%)
36.1
Number of peaks
18987 (qval < 1E-05)

hg38

Number of total reads
61955386
Reads aligned (%)
62.3
Duplicates removed (%)
34.8
Number of peaks
19055 (qval < 1E-05)

Base call quality data from DBCLS SRA