Antigen

Antigen Class

TFs and others

Antigen

E2F4

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

MCF10A-Er-Src

biomaterial_provider

Struhl laboratory

lab

Harvard

lab description

Struhl - Harvard University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

MCF10A-Er-Src

cell organism

human

cell description

mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)

cell karyotype

normal

cell lineage

ectoderm

cell sex

F

treatment

4OHTAM_1uM_36hr

treatment description

(To replace TAM_1uM_36hr) 36 h with 1 uM 4-hydroxytamoxifen (Myers)

antibody

E2F4

antibody antibodydescription

rabbit polyclonal. Antibody Target: E2F4

antibody targetdescription

mapping at the C-terminus of E2F4 of human origin

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-866

control

Harvard_Control

control description

input library was prepared at Harvard.

control

Harvard_Control

control description

input library was prepared at Harvard.

controlid

wgEncodeEH002871

replicate

1

Sequenced DNA Library

library_name

GSM935400: Harvard_ChipSeq_MCF10A-Er-Src_4OHTAM_1uM_36hr_E2F4_Harvard_Control

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

61955386

Reads aligned (%)

95.4

Duplicates removed (%)

34.8

Number of peaks

19055 (qval < 1E-05)

hg19

Number of total reads

61955386

Reads aligned (%)

94.5

Duplicates removed (%)

36.1

Number of peaks

18987 (qval < 1E-05)