Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NELFE

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
NELFe
antibody antibodydescription
NELFe (RDBP). Antibody Target: NELFe
antibody targetdescription
NELFe (RDBP) is a part of the negative elongation factor complex which binds to RNAPII to suppress elongation.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-32912
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000615
replicate
1

Sequenced DNA Library

library_name
GSM935392: Harvard_ChipSeq_K562_NELFe_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
38544300
Reads aligned (%)
52.6
Duplicates removed (%)
11.1
Number of peaks
1031 (qval < 1E-05)

hg38

Number of total reads
38544300
Reads aligned (%)
53.8
Duplicates removed (%)
9.9
Number of peaks
855 (qval < 1E-05)

Base call quality data from DBCLS SRA