Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

Pol2

antibody antibodydescription

mouse monoclonal, clone 8WG16. Antibody Target: POL2

antibody targetdescription

RNA Polymerase II

antibody vendorname

Covance

antibody vendorid

MMS-126R

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

controlid

wgEncodeEH000706

labversion

remapped from hg18, Illumina 2.0, low confidence sequences excluded. Data paired with GM12878_IgG_Control.

replicate

1

Sequenced DNA Library

library_name

GSM935386: Stanford_ChipSeq_GM12878_Pol2_IgG-mus

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

60061473

Reads aligned (%)

84.8

Duplicates removed (%)

10.1

Number of peaks

29791 (qval < 1E-05)

hg19

Number of total reads

60061473

Reads aligned (%)

83.6

Duplicates removed (%)

10.0

Number of peaks

30867 (qval < 1E-05)