Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RCOR1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
COREST_(ab24166)
antibody antibodydescription
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human CoREST. Note: This antibody has since been discontinued. Full product information is attached as part of validation document. Antibody Target: COREST_(ab24166)
antibody targetdescription
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates.
antibody vendorname
Abcam
antibody vendorid
ab24166
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935385: Stanford_ChipSeq_K562_COREST_(ab24166)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
56621578
Reads aligned (%)
90.9
Duplicates removed (%)
5.0
Number of peaks
9198 (qval < 1E-05)

hg38

Number of total reads
56621578
Reads aligned (%)
92.7
Duplicates removed (%)
3.8
Number of peaks
9335 (qval < 1E-05)

Base call quality data from DBCLS SRA