Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RFX5

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H1-hESC
cell organism
human
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
RFX5_(200-401-194)
antibody antibodydescription
Rabbit polyclonal against RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin. Antibody Target: RFX5
antibody targetdescription
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. RFX binds the X1 box of MHC-II promoters
antibody vendorname
Rockland Immunochemicals
antibody vendorid
200-401-194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001834
replicate
1

Sequenced DNA Library

library_name
GSM935382: Stanford_ChipSeq_H1-hESC_RFX5_(200-401-194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
69991065
Reads aligned (%)
79.5
Duplicates removed (%)
8.3
Number of peaks
2642 (qval < 1E-05)

hg38

Number of total reads
69991065
Reads aligned (%)
81.3
Duplicates removed (%)
7.0
Number of peaks
2950 (qval < 1E-05)

Base call quality data from DBCLS SRA