Antigen

Antigen Class

TFs and others

Antigen

RFX5

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1-hESC

biomaterial_provider

WiCell Research Institute

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

H1-hESC

cell organism

human

cell description

embryonic stem cells

cell karyotype

normal

cell lineage

inner cell mass

cell sex

M

treatment

None

treatment description

No special treatment or protocol applies

antibody

RFX5_(200-401-194)

antibody antibodydescription

Rabbit polyclonal against RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin. Antibody Target: RFX5

antibody targetdescription

Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. RFX binds the X1 box of MHC-II promoters

antibody vendorname

Rockland Immunochemicals

antibody vendorid

200-401-194

control

IgG-rab

control description

Input signal from Normal Rabbit IgG ChIP-seq.

control

IgG-rab

control description

Input signal from Normal Rabbit IgG ChIP-seq.

controlid

wgEncodeEH001834

replicate

1

Sequenced DNA Library

library_name

GSM935382: Stanford_ChipSeq_H1-hESC_RFX5_(200-401-194)_IgG-rab

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

69991065

Reads aligned (%)

87.4

Duplicates removed (%)

7.0

Number of peaks

2950 (qval < 1E-05)

hg19

Number of total reads

69991065

Reads aligned (%)

86.7

Duplicates removed (%)

8.3

Number of peaks

2642 (qval < 1E-05)