Antigen

Antigen Class

TFs and others

Antigen

SMC3

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Stanford

lab description

Snyder - Stanford University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM12878

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

treatment

None

treatment description

No special treatment or protocol applies

antibody

SMC3_(ab9263)

antibody antibodydescription

Rabbit polyclonal raised against synthetic peptide representing a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-3 conjugated to KLH. Antibody Target: SMC3

antibody targetdescription

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication.

antibody vendorname

Abcam

antibody vendorid

ab9263

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

control

IgG-mus

control description

Input signal from Normal Mouse IgG ChIP-seq.

controlid

wgEncodeEH000706

replicate

1

Sequenced DNA Library

library_name

GSM935376: Stanford_ChipSeq_GM12878_SMC3_(ab9263)_IgG-mus

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

73782483

Reads aligned (%)

91.0

Duplicates removed (%)

5.8

Number of peaks

33201 (qval < 1E-05)

hg19

Number of total reads

73782483

Reads aligned (%)

90.1

Duplicates removed (%)

6.8

Number of peaks

34887 (qval < 1E-05)