Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
NB-4
Primary Tissue
Blood
Site of Extraction
Bone Marrow
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
NB4
biomaterial_provider
Refer to protocol documents for differing sources, UW: DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) ACC-207 http://www.dsmz.de/ or Yale (Stanford): National Cell Culture Center http://www.nccc.com/
lab
Yale
lab description
Snyder - Yale University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
NB4
cell organism
human
cell description
acute promyelocytic leukemia cell line. (PMID: 1995093)
cell karyotype
cancer
cell lineage
mesoderm
cell sex
U
treatment
None
treatment description
No special treatment or protocol applies
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000617
replicate
1

Sequenced DNA Library

library_name
GSM935354: Yale_ChipSeq_NB4_Pol2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
18011814
Reads aligned (%)
88.9
Duplicates removed (%)
13.5
Number of peaks
20629 (qval < 1E-05)

hg19

Number of total reads
18011814
Reads aligned (%)
88.2
Duplicates removed (%)
13.6
Number of peaks
20629 (qval < 1E-05)

Base call quality data from DBCLS SRA