Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ELK1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
ELK1_(1277-1)
antibody antibodydescription
Rabbit polyclonal, a synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members. Antibody Target: ELK1
antibody targetdescription
Elk-1 is a transcription factor that is part of the ets oncogene superfamily. Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors. Activation by several mitogen- activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli.
antibody vendorname
Epitomics
antibody vendorid
1277-1
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000744
replicate
1

Sequenced DNA Library

library_name
GSM935326: Stanford_ChipSeq_HeLa-S3_ELK1_(1277-1)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
59572672
Reads aligned (%)
89.5
Duplicates removed (%)
5.9
Number of peaks
6840 (qval < 1E-05)

hg38

Number of total reads
59572672
Reads aligned (%)
91.4
Duplicates removed (%)
4.6
Number of peaks
6645 (qval < 1E-05)

Base call quality data from DBCLS SRA