Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SREBF2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Yale
lab description
Snyder - Yale University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
pravastatin
treatment description
For sterol deprivation, cells were cultured with pravastatin (2 uM, Sigma) in DMEM with 0.5% BSA for 16 h. (Snyder)
antibody
SREBP2
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SREBP2
antibody targetdescription
Sterol regulatory element binding transcription factor 2
antibody vendorname
Cayman Chemical
antibody vendorid
10007663
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000640
labversion
remapped from hg18
replicate
1

Sequenced DNA Library

library_name
GSM935314: Yale_ChipSeq_HepG2_pravastatin_SREBP2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
12004579
Reads aligned (%)
88.5
Duplicates removed (%)
3.0
Number of peaks
571 (qval < 1E-05)

hg38

Number of total reads
12004579
Reads aligned (%)
89.9
Duplicates removed (%)
2.5
Number of peaks
428 (qval < 1E-05)

Base call quality data from DBCLS SRA